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Rat CYP3A1BLR Bactosomes

Catalogue Number: CYP376

Product Description: Rat CYP3A1 and rat CYP-reductase coexpressed in Escherichia coli and supplemented with purified rat cytochrome b5

Amount: 1 nmol cytochrome P450

Storage Buffer: 50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA

Storage: Store at -80°C. Avoid frequent temperature changes. Thaw on ice.

Intended Use: For laboratory (research) purposes only

Typical Batch Characteristics

P450 Concentration: 2 nmol/ml

Protein Concentration: 10 mg/ml

Cytochrome b5 Concentration: 10 nmol/ml

Specific P450 Content: 200 pmol/mg protein

Cytochrome c Reductase Activity: 1400 nmol/min/mg protein

Typical P450 Activity Data

Activity: Testosterone 6β-hydroxylase

Linear with time up to: 8 min

Linear with P450 up to: 80 pmol/ml

Vmax: 3 pmol/min/pmol CYP

Km: 80 µM

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