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Rat CYP2D4LR Bactosomes

Catalogue Number: CYP361

Product Description: Rat CYP2D4 and rat CYP-reductase coexpressed in Escherichia coli

Amount: 1 nmol cytochrome P450

Storage Buffer: 50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA

Storage: Store at -80°C. Avoid frequent temperature changes. Thaw on ice.

Intended Use: For laboratory (research) purposes only

Typical Batch Characteristics

P450 Concentration: 3 nmol/ml

Protein Concentration: 12 mg/ml

Specific P450 Content: 250 pmol/mg protein

Cytochrome c Reductase Activity: 1250 nmol/min/mg protein

Typical P450 Activity Data

Activity: Dextromethorphan O-demethylase

Linear with time up to: 5 min

Linear with P450 up to: 25 pmol/ml

Vmax: 3 pmol/min/pmol CYP

Km: 15 µM

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