Catalogue Number: CYP370
Product Description: Rat CYP2B1 and rat CYP-reductase coexpressed in Escherichia coli and supplemented with purified rat cytochrome b5
Amount: 1 nmol cytochrome P450
Storage Buffer: 50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA
Storage: Store at -80°C. Avoid frequent temperature changes. Thaw on ice.
Intended Use: For laboratory (research) purposes only
Typical Batch Characteristics
P450 Concentration: 3 nmol/ml
Protein Concentration: 15 mg/ml
Cytochrome b5 Concentration: 15 nmol/ml
Specific P450 Content: 200 pmol/mg protein
Cytochrome c Reductase Activity: 1500 nmol/min/mg protein
Typical P450 Activity Data
Activity: Testosterone 16α-hydroxylase
Linear with time up to: 12 min
Linear with P450 up to: 20 pmol/ml
Vmax: 25 pmol/min/pmol CYP
Km: 20 – 30 µM