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Rat CYP1B1LR Bactosomes

Catalogue Number: CYP363

Product Description: Rat CYP1B1 and rat CYP-reductase coexpressed in Escherichia coli

Amount: 1 nmol cytochrome P450

Storage Buffer: 50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA

Storage: Store at -80°C. Avoid frequent temperature changes. Thaw on ice.

Intended Use: For laboratory (research) purposes only

Typical Batch Characteristics

P450 Concentration: 1.5 nmol/ml

Protein Concentration: 10 mg/ml

Specific P450 Content:
150 pmol/mg protein

Cytochrome c Reductase Activity:
1200 nmol/min/mg protein

Typical P450 Activity Data

Activity: 7-ethoxyresorufin O-deethylase

Linear with time up to: 5 min

Linear with P450 up to: 6 pmol/ml

Vmax: 1.5 pmol/min/pmol CYP

Km: 0.1 µM

Note: Incubation time 60s for P450 linearity test. Formation of resorufin deviates significantly from linearity at P450 concentrations less than 1 pmol/ml.

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