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Rat CYP1A2LR Bactosomes

Catalogue Number: CYP364

Product Description: Rat CYP1A2 and rat CYP-reductase coexpressed in Escherichia coli

Amount: 1 nmol cytochrome P450

Storage Buffer: 50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA

Storage: Store at -80°C. Avoid frequent temperature changes. Thaw on ice.

Intended Use: For laboratory (research) purposes only

Typical Batch Characteristics

P450 Concentration: 10 nmol/ml

Protein Concentration: 16 mg/ml

Specific P450 Content: 625 pmol/mg protein

Cytochrome c Reductase Activity: 2100 nmol/min/mg protein

Typical P450 Activity Data

Activity: 7-Ethoxyresorufin O-deethylase

Linear with time up to: 90 sec

Linear with P450 up to: 25 pmol/ml

Vmax: 8 pmol/min/pmol CYP

Km: 0.05 µM

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