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Pig CYP3A39BR Bactosomes

Catalogue Number: CYP456

Product Description: Pig CYP3A39 and pig CYP-reductase coexpressed in Escherichia coli and supplemented with purified human cytochrome b5

Amount: 1 nmol cytochrome P450

Storage Buffer: 50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA

Storage: Store at -80°C. Avoid frequent temperature changes. Thaw on ice.

Intended Use: For laboratory (research) purposes only

Typical Batch Characteristics

P450 Concentration: 2.4 nmol/ml

Protein Concentration: 12 mg/ml

Cytochrome b5 Concentration:
12 nmol/ml

Specific P450 Content:
200 pmol/mg protein

Cytochrome c Reductase Activity:
1500 nmol/min/mg protein

Typical P450 Activity Data

Activity: Testosterone 6β-hydroxylase

Linear with time up to: 4 min

Linear with P450 up to: 40 pmol/ml

Vmax: 20 pmol/min/pmol CYP

Km: 40 µM

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