Catalogue Number: CYP209
Product Description: Sf9 insect cell microsomes containing human UGT2B7.1 (His-268)
Amount: 2.5 mg protein (0.5 ml)
Storage Buffer: 25 mM Tris-acetate (pH 7.6), 10% (v/v) glycerol, 1 mM EDTA
Storage: Store at -80°C. Avoid frequent temperature changes. Thaw on ice.
Intended Use: For laboratory (research) purposes only
Typical Batch Characteristics
Protein Concentration: 5 mg/ml
Typical UGT Activity Data (1)
Substrate: 17β-estradiol (17-glucuronide formation)
Linear with time up to: 30 min
Linear with protein up to: 500 µg/ml
Vmax: 20 pmol/min/mg protein (without alamethicin)
Km (17β-estradiol): 2 – 3 µM
Typical UGT Activity Data (2)
Substrate: 4-methylumbelliferone
Linear with time up to: 30 min
Linear with protein up to: 500 µg/ml
Activity: 3000 – 4000 pmol/min/mg protein (at 1 mM 4-MU; without alamethicin)
S50 (4-MU): 400 – 500 µM