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Human UGT2B7

Catalogue Number: CYP209

Product Description: Sf9 insect cell microsomes containing human UGT2B7.1 (His-268)

Amount: 2.5 mg protein (0.5 ml)

Storage Buffer: 25 mM Tris-acetate (pH 7.6), 10% (v/v) glycerol, 1 mM EDTA

Storage: Store at -80°C. Avoid frequent temperature changes. Thaw on ice.

Intended Use: For laboratory (research) purposes only

Typical Batch Characteristics

Protein Concentration: 5 mg/ml

Typical UGT Activity Data (1)

Substrate: 17β-estradiol (17-glucuronide formation)

Linear with time up to: 30 min

Linear with protein up to: 500 µg/ml

Vmax: 20 pmol/min/mg protein (without alamethicin)

Km (17β-estradiol): 2 – 3 µM

Typical UGT Activity Data (2)

Substrate: 4-methylumbelliferone

Linear with time up to: 30 min

Linear with protein up to: 500 µg/ml

Activity: 3000 – 4000 pmol/min/mg protein (at 1 mM 4-MU; without alamethicin)

S50 (4-MU): 400 – 500 µM

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