Catalogue Number: CYP200
Product Description: Sf9 insect cell microsomes containing human UGT1A6
Amount: 2.5 mg protein (0.5 ml)
Storage Buffer: 25 mM Tris-acetate (pH 7.6), 10% (v/v) glycerol, 1 mM EDTA
Storage: Store at -80°C. Avoid frequent temperature changes. Thaw on ice.
Intended Use: For laboratory (research) purposes only
Typical Batch Characteristics
Protein Concentration: 5 mg/ml
Typical UGT Activity Data (1)
Substrate: 1-naphthol
Linear with time up to: 30 min
Linear with protein up to: 25 µg/ml
Vmax: 3000 pmol/min/mg protein
Km (1-naphthol): 1.8 – 5 µM
Typical UGT Activity Data (2)
Substrate: 4-methylumbelliferone (4MU)
Linear with time up to: 15 min
Linear with protein concentration up to: 200 µg/ml
Vmax: 15,000 pmol/min/mg protein
Km (4MU): About 125 µM