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Human UGT1A6 (E.coli-expressed)

Important Note: This product has now been superseded by UGT1A6 expressed in insect cells (details here). We will continue to hold stocks of the E. coli-expressed enzyme for a short period – if you wish to order this product, please be sure to specify the E. coli-expressed enzyme on your purchase order. Thank you.

Catalogue Number: CYP200

Product Description: Human UGT1A6 expressed in Escherichia coli

Amount: 5 mg protein

Storage Buffer: 50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA

Storage: Store at -80°C. Avoid frequent temperature changes. Thaw on ice.

Intended Use: For laboratory (research) purposes only

Typical Batch Characteristics

Protein Concentration: 10 mg/ml

Typical UGT Activity Data (1)

Substrate: 1-naphthol

Linear with time up to: 10 min

Linear with protein up to: 125 µg/ml

Vmax: 2.5 pmol/min/µg protein

Km (1-naphthol): 45 µM

Typical UGT Activity Data (2)

Substrate: 4-methylumbelliferone

Linear with time up to: 15 min

Linear with protein up to: 200 µg/ml

Vmax: 1.8 pmol/min/µg protein

Km (4-MU): 300 µM

Note: The Km (UDPGA) for the UGT1A6 is approximately 350 µM.

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