Important Note: This product has now been superseded by UGT1A6 expressed in insect cells (details here). We will continue to hold stocks of the E. coli-expressed enzyme for a short period – if you wish to order this product, please be sure to specify the E. coli-expressed enzyme on your purchase order. Thank you.
Catalogue Number: CYP200
Product Description: Human UGT1A6 expressed in Escherichia coli
Amount: 5 mg protein
Storage Buffer: 50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA
Storage: Store at -80°C. Avoid frequent temperature changes. Thaw on ice.
Intended Use: For laboratory (research) purposes only
Typical Batch Characteristics
Protein Concentration: 10 mg/ml
Typical UGT Activity Data (1)
Linear with time up to: 10 min
Linear with protein up to: 125 µg/ml
Vmax: 2.5 pmol/min/µg protein
Km (1-naphthol): 45 µM
Typical UGT Activity Data (2)
Linear with time up to: 15 min
Linear with protein up to: 200 µg/ml
Vmax: 1.8 pmol/min/µg protein
Km (4-MU): 300 µM
Note: The Km (UDPGA) for the UGT1A6 is approximately 350 µM.