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Human UGT1A1

Catalogue Number: CYP201

Product Description: Sf9 insect cell microsomes containing human UGT1A1

Amount: 2.5 mg protein (0.5 ml)

Storage Buffer: 25 mM Tris-acetate (pH 7.6), 10% (v/v) glycerol, 1 mM EDTA

Storage: Store at -80°C. Avoid frequent temperature changes. Thaw on ice.

Intended Use: For laboratory (research) purposes only

Typical Batch Characteristics

Protein Concentration: 5 mg/ml

Typical UGT Activity Data (1)

Substrate: 17β-estradiol (3-glucuronide formation)

Linear with time up to: 15 min

Linear with protein up to: 500 µg/ml

Vmax: 700 pmol/min/mg protein

S50 (17β-estradiol): 10 – 15 µM

Typical UGT Activity Data (2)

Substrate: Bilirubin

Activity: 400 – 500 pmol/min/mg protein (at 2 µM bilirubin)

Typical UGT Activity Data (3)

Substrate: 4-methylumbelliferone

Activity: 300 – 400 pmol/min/mg protein (at 80 µM 4MU)

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