Catalogue Number: CYP201
Product Description: Sf9 insect cell microsomes containing human UGT1A1
Amount: 2.5 mg protein (0.5 ml)
Storage Buffer: 25 mM Tris-acetate (pH 7.6), 10% (v/v) glycerol, 1 mM EDTA
Storage: Store at -80°C. Avoid frequent temperature changes. Thaw on ice.
Intended Use: For laboratory (research) purposes only
Typical Batch Characteristics
Protein Concentration: 5 mg/ml
Typical UGT Activity Data (1)
Substrate: 17β-estradiol (3-glucuronide formation)
Linear with time up to: 15 min
Linear with protein up to: 500 µg/ml
Vmax: 700 pmol/min/mg protein
S50 (17β-estradiol): 10 – 15 µM
Typical UGT Activity Data (2)
Substrate: Bilirubin
Activity: 400 – 500 pmol/min/mg protein (at 2 µM bilirubin)
Typical UGT Activity Data (3)
Substrate: 4-methylumbelliferone
Activity: 300 – 400 pmol/min/mg protein (at 80 µM 4MU)