Catalogue Number: CYP305
Product Description: Dog CYP3A12 and dog CYP-reductase coexpressed in Escherichia coli
Amount: 1 nmol cytochrome P450
Storage Buffer: 50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA
Storage: Store at -80°C. Avoid frequent temperature changes. Thaw on ice.
Intended Use: For laboratory (research) purposes only
Typical Batch Characteristics
P450 Concentration: 8.0 nmol/ml
Protein Concentration: 25 mg/ml
Specific P450 Content: 320 pmol/mg protein
Cytochrome c Reductase Activity: 480 nmol/min/mg protein
Typical P450 Activity Data
Activity: Testosterone 6β-hydroxylase
Linear with time up to: 20 min
Linear with P450 up to: 60 pmol/ml
Vmax: 10 pmol/min/pmol CYP
Km: 50 µM