Catalogue Number: CYP304
Product Description: Dog CYP2D15 and dog CYP-reductase coexpressed in Escherichia coli
Amount: 1 nmol cytochrome P450
Storage Buffer: 50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA
Storage: Store at -80°C. Avoid frequent temperature changes. Thaw on ice.
Intended Use: For laboratory (research) purposes only
Typical Batch Characteristics
P450 Concentration: 2 nmol/ml
Protein Concentration: 10 mg/ml
Specific P450 Content:
200 pmol/mg protein
Cytochrome c Reductase Activity:
400 nmol/min/mg protein
Typical P450 Activity Data (1)
Activity: Bufuralol 1′-hydroxylase
Linear with time up to: 5 min
Linear with P450 up to: 8 pmol/ml
Vmax: 7 pmol/min/pmol CYP
Km: 5 µM
Typical P450 Activity Data (2)
Activity: Dextromethorphan O-demethylase
Vmax: 18 pmol/min/pmol CYP
Km: 0.8 µM
Note: This reaction is only linear with incubation time for approximately 1 min.