Catalogue Number: CYP303
Product Description: Dog CYP2C41 and dog CYP-reductase coexpressed in Escherichia coli
Amount: 1 nmol cytochrome P450
Storage Buffer: 50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA
Storage: Store at -80°C. Avoid frequent temperature changes. Thaw on ice.
Intended Use: For laboratory (research) purposes only
Typical Batch Characteristics
P450 Concentration: 7.5 nmol/ml
Protein Concentration: 25 mg/ml
Specific P450 Content: 300 pmol/mg protein
Cytochrome c Reductase Activity: 600 nmol/min/mg protein
Typical P450 Activity Data (1)
Activity: Diclofenac 4′-hydroxylase
Linear with time up to: 60 min
Linear with P450 up to: 150 pmol/ml
Vmax: 0.25 pmol/min/pmol CYP
Km: 60 µM
Typical P450 Activity Data (2)
Activity: Tolbutamide 4-hydroxylase
Linear with time up to: 60 min
Linear with P450 up to: 100 pmol/ml
Vmax: 2 pmol/min/pmol CYP
Km: 1700 µM (see note below)
Note: The kinetic parameters given above were determined using tolbutamide concentrations from 40 – 500 µM, as the limit of solubility is reported to be around 740 µM. The margin of error is therefore likely to be high. The tolbutamide 4-hydroxylase activity of CYP2C41LR Bactosomes at 500 µM tolbutamide is approximately 0.5 pmol/min/pmol CYP.