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Dog CYP2C41LR Bactosomes

Catalogue Number: CYP303

Product Description: Dog CYP2C41 and dog CYP-reductase coexpressed in Escherichia coli

Amount: 1 nmol cytochrome P450

Storage Buffer: 50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA

Storage: Store at -80°C. Avoid frequent temperature changes. Thaw on ice.

Intended Use: For laboratory (research) purposes only

Typical Batch Characteristics

P450 Concentration: 7.5 nmol/ml

Protein Concentration: 25 mg/ml

Specific P450 Content: 300 pmol/mg protein

Cytochrome c Reductase Activity: 600 nmol/min/mg protein

Typical P450 Activity Data (1)

Activity: Diclofenac 4′-hydroxylase

Linear with time up to: 60 min

Linear with P450 up to: 150 pmol/ml

Vmax: 0.25 pmol/min/pmol CYP

Km: 60 µM

Typical P450 Activity Data (2)

Activity: Tolbutamide 4-hydroxylase

Linear with time up to: 60 min

Linear with P450 up to: 100 pmol/ml

Vmax: 2 pmol/min/pmol CYP

Km: 1700 µM (see note below)

Note: The kinetic parameters given above were determined using tolbutamide concentrations from 40 – 500 µM, as the limit of solubility is reported to be around 740 µM. The margin of error is therefore likely to be high. The tolbutamide 4-hydroxylase activity of CYP2C41LR Bactosomes at 500 µM tolbutamide is approximately 0.5 pmol/min/pmol CYP.

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