Catalogue Number: CYP302
Product Description: Dog CYP2C21 and dog CYP-reductase coexpressed in Escherichia coli
Amount: 1 nmol cytochrome P450
Storage Buffer: 50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA
Storage: Store at -80°C. Avoid frequent temperature changes. Thaw on ice.
Intended Use: For laboratory (research) purposes only
Typical Batch Characteristics
P450 Concentration: 2 nmol/ml
Protein Concentration: 10 mg/ml
Specific P450 Content: 200 pmol/mg protein
Cytochrome c Reductase Activity: 400 nmol/min/mg protein
Typical P450 Activity Data (1)
Activity: Diclofenac 4′-hydroxylase
Linear with time up to: 10 min
Linear with P450 up to: 125 pmol/ml
Vmax: 2.5 pmol/min/pmol CYP
Km: 35 µM
Typical P450 Activity Data (2)
Activity: Tolbutamide 4-hydroxylase
Note: Data obtained over a substrate concentration range from 40 – 500 µM do not fit to the Michaelis-Menten equation. The tolbutamide 4-hydroxylase activity of CYP2C21LR Bactosomes at 500 µM tolbutamide is approximately 0.2 pmol/min/pmol CYP. This is lower than the activity of CYP2C41LR Bactosomes at the same substrate concentration.