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Dog CYP2C21LR Bactosomes

Catalogue Number: CYP302

Product Description: Dog CYP2C21 and dog CYP-reductase coexpressed in Escherichia coli

Amount: 1 nmol cytochrome P450

Storage Buffer: 50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA

Storage: Store at -80°C. Avoid frequent temperature changes. Thaw on ice.

Intended Use: For laboratory (research) purposes only

Typical Batch Characteristics

P450 Concentration: 2 nmol/ml

Protein Concentration: 10 mg/ml

Specific P450 Content: 200 pmol/mg protein

Cytochrome c Reductase Activity: 400 nmol/min/mg protein

Typical P450 Activity Data (1)

Activity: Diclofenac 4′-hydroxylase

Linear with time up to: 10 min

Linear with P450 up to: 125 pmol/ml

Vmax: 2.5 pmol/min/pmol CYP

Km: 35 µM

Typical P450 Activity Data (2)

Activity: Tolbutamide 4-hydroxylase

Note: Data obtained over a substrate concentration range from 40 – 500 µM do not fit to the Michaelis-Menten equation. The tolbutamide 4-hydroxylase activity of CYP2C21LR Bactosomes at 500 µM tolbutamide is approximately 0.2 pmol/min/pmol CYP. This is lower than the activity of CYP2C41LR Bactosomes at the same substrate concentration.

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