Catalogue Number: CYP301
Product Description: Dog CYP2B11 and dog CYP-reductase coexpressed in Escherichia coli
Amount: 1 nmol cytochrome P450
Storage Buffer: 50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA
Storage: Store at -80°C. Avoid frequent temperature changes. Thaw on ice.
Intended Use: For laboratory (research) purposes only
Typical Batch Characteristics
P450 Concentration: 3.6 nmol/ml
Protein Concentration: 12 mg/ml
Specific P450 Content: 300 pmol/mg protein
Cytochrome c Reductase Activity: 900 nmol/min/mg protein
Typical P450 Activity Data (1)
Activity: Diazepam N-demethylase
Linear with time up to: 10 min
Linear with P450 up to: 6 pmol/ml
Vmax: 15 pmol/min/pmol CYP
Km: 10 µM
Typical P450 Activity Data (2)
Activity: Bupropion hydroxylase
Linear with time up to: 5 min
Linear with P450 up to: 30 pmol/ml
Vmax: 2 – 3 pmol/min/pmol CYP
Km: 35 – 50 µM