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Dog CYP1B1LR Bactosomes

Catalogue Number: CYP308

Product Description: Dog CYP1B1 and dog CYP-reductase coexpressed in Escherichia coli

Amount: 1 nmol cytochrome P450

Storage Buffer: 50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA

Storage: Store at -80°C. Avoid frequent temperature changes. Thaw on ice.

Intended Use: For laboratory (research) purposes only

Typical Batch Characteristics

P450 Concentration: 6 nmol/ml

Protein Concentration: 15 mg/ml

Specific P450 Content: 400 pmol/mg protein

Cytochrome c Reductase Activity: 1000 nmol/min/mg protein

Typical P450 Activity Data

Activity: 7-ethoxyresorufin O-deethylase

Linear with time up to: 1.5 min

Linear with P450 up to: 2 pmol/ml

Vmax: 40 pmol/min/pmol CYP

Km: 0.04 µM

Note: Incubation time 10s for P450 linearity test; 15s for kinetics test.

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