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CYP4F3BR Bactosomes

Catalogue Number: CYP056

Product Description: Human CYP4F3B and human CYP-reductase coexpressed in Escherichia coli

Amount: 1 nmol cytochrome P450

Storage Buffer: 50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA

Storage: Store at -80°C. Avoid frequent temperature changes. Thaw on ice.

Intended Use: For laboratory (research) purposes only

Note: This product is also available in our convenient EasyCYP format.

Typical Batch Characteristics

P450 Concentration: 3 nmol/ml

Protein Concentration: 15 mg/ml

Specific P450 Content:
200 pmol/mg protein

Cytochrome c Reductase Activity:
800 nmol/min/mg protein

Typical P450 Activity Data

Activity: Leukotriene B4 ω-hydroxylase

Linear with time up to: 10 min

Linear with P450 up to: 20 pmol/ml

Vmax: 6 pmol/min/pmol CYP

Km: 2 µM (see note below)

Note: The Km for CYP4F3B expressed in E. coli is approximately 10-fold lower than that reported for CYP4F3B expressed in insect cells. The reason for this is unknown.

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