Catalogue Number: CYP057
Product Description: Human CYP4F3B and human CYP-reductase coexpressed in Escherichia coli and supplemented with purified human cytochrome b5
Amount: 1 nmol cytochrome P450
Storage Buffer: 50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA
Storage: Store at -80°C. Avoid frequent temperature changes. Thaw on ice.
Intended Use: For laboratory (research) purposes only
Note: This product is also available in our convenient EasyCYP format.
Typical Batch Characteristics
P450 Concentration: 3 nmol/ml
Protein Concentration: 15 mg/ml
Cytochrome b5 Concentration:
Specific P450 Content:
200 pmol/mg protein
Cytochrome c Reductase Activity:
200 nmol/min/mg protein
Typical P450 Activity Data
Activity: Leukotriene B4 ω-hydroxylase
Linear with time up to: 20 min
Linear with P450 up to: 120 pmol/ml
Vmax: 0.8 pmol/min/pmol CYP
Km: 2 µM (see note below)
Note: The Km for CYP4F3B expressed in E. coli is approximately 10-fold lower than that reported for CYP4F3B expressed in insect cells. The reason for this is unknown.