Catalogue Number: CYP043
Product Description: Human CYP4A11 and human CYP-reductase coexpressed in Escherichia coli and supplemented with purified human cytochrome b5
Amount: 1 nmol cytochrome P450
Storage Buffer: 50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA
Storage: Store at -80°C. Avoid frequent temperature changes. Thaw on ice.
Intended Use: For laboratory (research) purposes only
Note: This product is also available in our convenient EasyCYP format.
Typical Batch Characteristics
P450 Concentration: 5.0 nmol/ml
Protein Concentration: 25 mg/ml
Cytochrome b5 Concentration:
25 nmol/ml
Specific P450 Content:
150 pmol/mg protein
Cytochrome c Reductase Activity:
750 nmol/min/mg protein
Typical P450 Activity Data
Activity: Lauric acid 12-hydroxylase
Linear with time up to: 8 min
Linear with P450 up to: 6 pmol/ml
Vmax: 20 pmol/min/pmol CYP
Km: 1.5 µM