Testosterone turnover by CYP3A5 is sensitive to the concentration of phosphate in the incubation.Catalogue Number: CYP/EZ045
Product Description: Human CYP3A5 and human CYP-reductase coexpressed in Escherichia coli and supplemented with purified human cytochrome b5
Amount: 0.5 nmol cytochrome P450
Storage Buffer: 50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA
Storage: Store at -80°C. Avoid frequent temperature changes. Thaw on ice.
Intended Use: For laboratory (research) purposes only
Typical Batch Characteristics
P450 Concentration: 1.0 nmol/ml
Protein Concentration: 10 mg/ml
Cytochrome b5 Concentration:
5 nmol/ml
Specific P450 Content:
100 pmol/mg protein
Cytochrome c Reductase Activity:
150 nmol/min/mg protein
Typical P450 Activity Data
Activity: Testosterone 6β-hydroxylase
Linear with time up to: 20 min
Linear with P450 up to: 60 pmol/ml
Vmax: 14 pmol/min/pmol CYP
Km: 70 µM