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CYP3A4BR Bactosomes

Catalogue Number: CYP005

Product Description: Human CYP3A4 and human CYP-reductase coexpressed in Escherichia coli and supplemented with purified human cytochrome b5

Amount: 1 nmol cytochrome P450

Storage Buffer: 50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA

Storage: Store at -80°C. Avoid frequent temperature changes. Thaw on ice.

Intended Use: For laboratory (research) purposes only

Note: This product is also available in our convenient EasyCYP format.

Typical Batch Characteristics

P450 Concentration: 4 nmol/ml

Protein Concentration: 12 mg/ml

Cytochrome b5 Concentration:
20 nmol/ml

Specific P450 Content:
330 pmol/mg protein

Cytochrome c Reductase Activity:
1600 nmol/min/mg protein

Typical P450 Activity Data

Activity: Testosterone 6β-hydroxylase

Linear with time up to: 6 min

Linear with P450 up to: 20 pmol/ml

Vmax: 75 pmol/min/pmol CYP

Km: 25 µM

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