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CYP2D6R EasyCYP Bactosomes

Catalogue Number: CYP/EZ007

Product Description: Human CYP2D6 and human CYP-reductase coexpressed in Escherichia coli

Amount: 0.5 nmol cytochrome P450

Storage Buffer: 50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA

Storage: Store at -80°C. Avoid frequent temperature changes. Thaw on ice.

Intended Use: For laboratory (research) purposes only.

Typical Batch Characteristics

P450 Concentration: 1.0 nmol/ml

Protein Concentration: 10 mg/ml

Specific P450 Content:
100 pmol/mg protein

Cytochrome c Reductase Activity:
1000 nmol/min/mg protein

Typical P450 Activity Data (1)

Activity: Dextromethorphan O-demethylase

Linear with time up to: 8 min

Linear with P450 up to: 4 pmol/ml

Vmax: 12 pmol/min/pmol CYP

Km: 0.3 µM

Typical P450 Activity Data (2)

Activity: Bufuralol 1′-hydroxylase

Linear with time up to: 4 min

Linear with P450 up to: 3 pmol/ml

Vmax: 50 pmol/min/pmol CYP

Km: 2 µM

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