Cypex Logo

CYP2D6*10R EasyCYP Bactosomes

Catalogue Number: CYP/EZ029

Product Description: Human CYP2D6.10 and human CYP-reductase coexpressed in Escherichia coli

Amount: 0.5 nmol cytochrome P450

Storage Buffer: 50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA

Storage: Store at -80°C. Avoid frequent temperature changes. Thaw on ice.

Intended Use: For laboratory (research) purposes only

Typical Batch Characteristics

P450 Concentration: 1.0 nmol/ml

Protein Concentration: 10 mg/ml

Specific P450 Content:
100 pmol/mg protein

Cytochrome c Reductase Activity:
1500 nmol/min/mg protein

Typical P450 Activity Data (1)

Activity: Dextromethorphan O-demethylase

Linear with time up to: 5 min

Linear with P450 up to: 30 pmol/ml

Vmax: 10 pmol/min/pmol CYP

Km: 7 µM

Typical P450 Activity Data (2)

Activity: Bufuralol 1′-hydroxylase

Linear with time up to: 4 min

Linear with P450 up to: 8 pmol/ml

Vmax: 20 pmol/min/pmol CYP

Km: 10 µM

Latest Blogs