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CYP2D6*10R Bactosomes

Catalogue Number: CYP029

Product Description: Human CYP2D6.10 and human CYP-reductase coexpressed in Escherichia coli

Amount: 1 nmol cytochrome P450

Storage Buffer: 50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA

Storage: Store at -80°C. Avoid frequent temperature changes. Thaw on ice.

Intended Use: For laboratory (research) purposes only

Note: This product is also available in our convenient EasyCYP format.

Typical Batch Characteristics

P450 Concentration: 1.6 nmol/ml

Protein Concentration: 16 mg/ml

Specific P450 Content:
100 pmol/mg protein

Cytochrome c Reductase Activity:
1700 nmol/min/mg protein

Typical P450 Activity Data (1)

Activity: Dextromethorphan O-demethylase

Linear with time up to: 6 min

Linear with P450 up to: 30 pmol/ml

Vmax: 12 pmol/min/pmol CYP

Km: 8 µM

Typical P450 Activity Data (2)

Activity: Bufuralol 1′-hydroxylase

Linear with time up to: 4 min

Linear with P450 up to: 8 pmol/ml

Vmax: 20 pmol/min/pmol CYP

Km: 10 µM

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