Catalogue Number: CYP017
Product Description: Human CYP2C8 and human CYP-reductase coexpressed in Escherichia coli
Amount: 1 nmol cytochrome P450
Storage Buffer: 50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA
Storage: Store at -80°C. Avoid frequent temperature changes. Thaw on ice.
Intended Use: For laboratory (research) purposes only
Note: This product is also available in our convenient EasyCYP format.
Typical Batch Characteristics
P450 Concentration: 10 nmol/ml
Protein Concentration: 8 mg/ml
Specific P450 Content:
1250 pmol/mg protein
Cytochrome c Reductase Activity:
2500 nmol/min/mg protein
Typical P450 Activity Data (1)
Activity: Paclitaxel 6α-hydroxylase
Linear with time up to: 8 min
Linear with P450 up to: 60 pmol/ml
Vmax: 5 pmol/min/pmol CYP
Km: 4 µM
Typical P450 Activity Data (2)
Activity: Tolbutamide 4-hydroxylase
Linear with time up to: 12 min
Linear with P450 up to:
100 pmol/ml
Vmax: 3 pmol/min/pmol CYP
Km: 1100 µM (see note below)
Note: The kinetic parameters given above were determined using tolbutamide concentrations from 40 – 500 µM, as the limit of solubility is reported to be around 740 µM. The margin of error is therefore likely to be high. The tolbutamide 4-hydroxylase activity of CYP2C8R Bactosomes at 500 µM tolbutamide is approximately 1.0 pmol/min/pmol CYP.