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CYP2C8BR Bactosomes

Catalogue Number: CYP049

Product Description: Human CYP2C8 and human CYP-reductase coexpressed in Escherichia coli and supplemented with purified human cytochrome b5

Amount: 1 nmol cytochrome P450

Storage Buffer: 50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA

Storage: Store at -80°C. Avoid frequent temperature changes. Thaw on ice.

Intended Use: For laboratory (research) purposes only

Note: This product is also available in our convenient EasyCYP format.

Typical Batch Characteristics

P450 Concentration: 6 nmol/ml

Protein Concentration: 12 mg/ml

Cytochrome b5 Concentration:
30 nmol/ml

Specific P450 Content:
500 pmol/mg protein

Cytochrome c Reductase Activity:
800 nmol/min/mg protein

Typical P450 Activity Data (1)

Activity: Paclitaxel 6α-hydroxylase

Linear with time up to: 6 min

Linear with P450 up to: 40 pmol/ml

Vmax: 5 pmol/min/pmol CYP

Km: 4 µM

Typical P450 Activity Data (2)

Activity: Tolbutamide 4-hydroxylase

Linear with time up to: 12 min

Linear with P450 up to:
100 pmol/ml

Vmax: 3 pmol/min/pmol CYP

Km: 1100 µM (see note below)

Note: The kinetic parameters given above were determined using tolbutamide concentrations from 40 – 500 µM, as the limit of solubility is reported to be around 740 µM. The margin of error is therefore likely to be high. The tolbutamide 4-hydroxylase activity of CYP2C8BR Bactosomes at 500 µM tolbutamide is approximately 1.2 pmol/min/pmol CYP.

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