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CYP2C19R Bactosomes

Catalogue Number: CYP008

Product Description: Human CYP2C19 and human CYP-reductase coexpressed in Escherichia coli

Amount: 1 nmol cytochrome P450

Storage Buffer: 50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA

Storage: Store at -80°C. Avoid frequent temperature changes. Thaw on ice.

Intended Use: For laboratory (research) purposes only

Note: This product is also available in our convenient EasyCYP format.

Typical Batch Characteristics

P450 Concentration: 4.5 nmol/ml

Protein Concentration: 10 mg/ml

Specific P450 Content:
450 pmol/mg protein

Cytochrome c Reductase Activity:
1800 nmol/min/mg protein

Typical P450 Activity Data (1)

Activity: Diazepam N-demethylase

Linear with time up to: 12 min

Linear with P450 up to: 80 pmol/ml

Vmax: 3 pmol/min/pmol CYP

Km: 20 µM

Typical P450 Activity Data (2)

Activity: S-mephenytoin 4′-hydroxylase

Linear with time up to: 8 min

Linear with P450 up to: 80 pmol/ml

Vmax: 10 pmol/min/pmol CYP

Km: 45 µM

Typical P450 Activity Data (3)

Activity: Tolbutamide 4-hydroxylase

Linear with time up to: 15 min

Linear with P450 up to: 50 pmol/ml

Vmax: 12 pmol/min/pmol CYP

Km: 200 µM

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