Catalogue Number: CYP028
Product Description: Human CYP2C19 and human CYP-reductase coexpressed in Escherichia coli
Amount: 1 nmol cytochrome P450
Storage Buffer: 50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA
Storage: Store at -80°C. Avoid frequent temperature changes. Thaw on ice.
Intended Use: For laboratory (research) purposes only
Note: This product is also available in our convenient EasyCYP format.
Typical Batch Characteristics
P450 Concentration: 5 nmol/ml
Protein Concentration: 12 mg/ml
Specific P450 Content:
420 pmol/mg protein
Cytochrome c Reductase Activity:
240 nmol/min/mg protein
Typical P450 Activity Data (1)
Activity: Diazepam N-demethylase
Linear with time up to: 20 min
Linear with P450 up to: 100 pmol/ml
Vmax: 0.6 pmol/min/pmol CYP
Km: 25 µM
Typical P450 Activity Data (2)
Activity: S-mephenytoin 4′-hydroxylase
Linear with time up to: 25 min
Linear with P450 up to: 150 pmol/ml
Vmax: 2.5 pmol/min/pmol CYP
Km: 45 µM
Typical P450 Activity Data (3)
Activity: Tolbutamide 4-hydroxylase
Linear with time up to: 30 min
Linear with P450 up to: 100 pmol/ml
Vmax: 3 pmol/min/pmol CYP
Km: 200 µM