Catalogue Number: CYP022
Product Description: Human CYP2C18 and human CYP-reductase coexpressed in Escherichia coli
Amount: 1 nmol cytochrome P450
Storage Buffer: 50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA
Storage: Store at -80°C. Avoid frequent temperature changes. Thaw on ice.
Intended Use: For laboratory (research) purposes only
Note: This product is also available in our convenient EasyCYP format.
Typical Batch Characteristics
P450 Concentration: 7 nmol/ml
Protein Concentration: 10 mg/ml
Specific P450 Content:
700 pmol/mg protein
Cytochrome c Reductase Activity:
500 nmol/min/mg protein
Typical P450 Activity Data (1)
Activity: Diclofenac 4′-hydroxylase
Linear with time up to: 6 min
Linear with P450 up to: 250 pmol/ml
Vmax: 0.5 pmol/min/pmol CYP
Km: 50 µM
Typical P450 Activity Data (2)
Activity: Tolbutamide 4-hydroxylase
Note: Data obtained over a substrate concentration range from 40 – 500 µM give a poor fit to the Michaelis-Menten equation. The tolbutamide 4-hydroxylase activity of CYP2C18LR Bactosomes at 500 µM tolbutamide is approximately 0.5 pmol/min/pmol CYP.