CYP2C18LR Bactosomes (low reductase)

Catalogue Number: CYP022

Product Description: Human CYP2C18 and human CYP-reductase coexpressed in Escherichia coli

Amount: 1 nmol cytochrome P450

Storage Buffer: 50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA

Storage: Store at -80°C. Avoid frequent temperature changes. Thaw on ice.

Intended Use: For laboratory (research) purposes only

Note: This product is also available in our convenient EasyCYP format.

Typical Batch Characteristics

P450 Concentration: 7 nmol/ml

Protein Concentration: 10 mg/ml

Specific P450 Content:
700 pmol/mg protein

Cytochrome c Reductase Activity:
500 nmol/min/mg protein

Typical P450 Activity Data (1)

Activity: Diclofenac 4′-hydroxylase

Linear with time up to: 6 min

Linear with P450 up to: 250 pmol/ml

Vmax: 0.5 pmol/min/pmol CYP

Km: 50 µM

Typical P450 Activity Data (2)

Activity: Tolbutamide 4-hydroxylase

Note: Data obtained over a substrate concentration range from 40 – 500 µM give a poor fit to the Michaelis-Menten equation. The tolbutamide 4-hydroxylase activity of CYP2C18LR Bactosomes at 500 µM tolbutamide is approximately 0.5 pmol/min/pmol CYP.