Catalogue Number: CYP020
Product Description: Human CYP2B6 and human CYP-reductase coexpressed in Escherichia coli
Amount: 1 nmol cytochrome P450
Storage Buffer: 50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA
Storage: Store at -80°C. Avoid frequent temperature changes. Thaw on ice.
Intended Use: For laboratory (research) purposes only
Note: This product is also available in our convenient EasyCYP format.
Typical Batch Characteristics
P450 Concentration: 2 nmol/ml
Protein Concentration: 10 mg/ml
Specific P450 Content:
200 pmol/mg protein
Cytochrome c Reductase Activity:
2000 nmol/min/mg protein
Typical P450 Activity Data (1)
Activity: Diazepam N-demethylase
Linear with time up to: 5 min
Linear with P450 up to: 20 pmol/ml
Vmax: 4 pmol/min/pmol CYP
Km: 70 µM
Typical P450 Activity Data (2)
Activity: Bupropion hydroxylase
Linear with time up to: 5 min
Linear with P450 up to: 20 pmol/ml
Vmax: 12 – 20 pmol/min/pmol CYP
Km: 55 µM
Typical P450 Activity Data (3)
Activity: 7-ethoxy-4-(trifluoromethyl)-
coumarin (EFC) O-deethylase
Linear with time up to: 6 min
Linear with P450 up to: 8 pmol/ml
Vmax: 3 – 5 pmol/min/pmol CYP
Km: 2 – 3.5 µM