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CYP2B6LR Bactosomes (low reductase)

Catalogue Number: CYP016

Product Description: Human CYP2B6 and human CYP-reductase coexpressed in Escherichia coli

Amount: 1 nmol cytochrome P450

Storage Buffer: 50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA

Storage: Store at -80°C. Avoid frequent temperature changes. Thaw on ice.

Intended Use: For laboratory (research) purposes only

Note: This product is also available in our convenient EasyCYP format.

Typical Batch Characteristics

P450 Concentration: 5.0 nmol/ml

Protein Concentration: 10 mg/ml

Specific P450 Content:
500 pmol/mg protein

Cytochrome c Reductase Activity:
500 nmol/min/mg protein

Typical P450 Activity Data (1)

Activity: Diazepam N-demethylase

Linear with time up to: 15 min

Linear with P450 up to: 100 pmol/ml

Vmax: 0.6 pmol/min/pmol CYP

Km: 130 µM

Typical P450 Activity Data (2)

Activity: Bupropion hydroxylase

Linear with time up to: 5 min

Linear with P450 up to: 50 pmol/ml

Vmax: 2 pmol/min/pmol CYP

Km: 90 – 120 µM

Typical P450 Activity Data (3)

Activity: 7-ethoxy-4-(trifluoromethyl)-
coumarin (EFC) O-deethylase

Linear with time up to: 15 min

Linear with P450 up to: 15 pmol/ml

Vmax: 0.7 pmol/min/pmol CYP

Km: 3.5 µM

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