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CYP2B6BR EasyCYP Bactosomes

Catalogue Number: CYP/EZ041

Product Description: Human CYP2B6 and human CYP-reductase coexpressed in Escherichia coli and supplemented with purified human cytochrome b5

Amount: 0.5 nmol cytochrome P450

Storage Buffer: 50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA

Storage: Store at -80°C. Avoid frequent temperature changes. Thaw on ice.

Intended Use: For laboratory (research) purposes only

Typical Batch Characteristics

P450 Concentration: 1 nmol/ml

Protein Concentration: 10 mg/ml

Cytochrome b5 Concentration:
5 nmol/ml

Specific P450 Content:
100 pmol/mg protein

Cytochrome c Reductase Activity:
1000 nmol/min/mg protein

Typical P450 Activity Data (1)

Activity: Diazepam N-demethylase

Linear with time up to: 5 min

Linear with P450 up to: 20 pmol/ml

Vmax: 5 pmol/min/pmol CYP

Km: 70 µM

Typical P450 Activity Data (2)

Activity: 7-ethoxy-4-(trifluoromethyl)-
coumarin (EFC) O-deethylase

Linear with time up to: 12 min

Linear with P450 up to: 5 pmol/ml

Vmax: 5 pmol/min/pmol CYP

Km: 2 – 3.5 µM

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