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CYP1B1*4LR Bactosomes (low reductase)

Catalogue Number: CYP024

Product Description: Human CYP1B1.4 (Ser-453) and human CYP-reductase coexpressed in Escherichia coli

Amount: 1 nmol cytochrome P450

Storage Buffer: 50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA

Storage: Store at -80°C. Avoid frequent temperature changes. Thaw on ice.

Intended Use: For laboratory (research) purposes only

Note: This product is also available in our convenient EasyCYP format.

Typical Batch Characteristics

P450 Concentration: 7.0 nmol/ml

Protein Concentration: 12.0 mg/ml

Specific P450 Content: 583 pmol/mg protein

Cytochrome c Reductase Activity: 150 nmol/min/mg protein

Typical P450 Activity Data (1)

Activity: 17β-estradiol 4-hydroxylase

Linear with time up to: 20 min

Linear with P450 up to: 60 pmol/ml

Vmax: 1 pmol/min/pmol CYP

Km: 0.8 µM

Typical P450 Activity Data (2)

Activity: 7-ethoxyresorufin O-deethylase

Linear with time up to: 5 min

Linear with P450 up to: 20 pmol/ml

Vmax: 6 pmol/min/pmol CYP

Km: 0.1 µM

Note: Incubation time 10s for P450 linearity test. Formation of resorufin deviates significantly from linearity at P450 concentrations less than 2 pmol/ml.

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