Catalogue Number: CYP/EZ001
Product Description: Human CYP1A2 and human CYP-reductase coexpressed in Escherichia coli
Amount: 0.5 nmol cytochrome P450
Storage Buffer: 50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA
Storage: Store at -80°C. Avoid frequent temperature changes. Thaw on ice.
Intended Use: For laboratory (research) purposes only
Typical Batch Characteristics
P450 Concentration: 1.0 nmol/ml
Protein Concentration: 10.0 mg/ml
Specific P450 Content: 100 pmol/mg protein
Cytochrome c Reductase Activity: 80 nmol/min/mg protein
Typical P450 Activity Data (1)
Activity: 7-ethoxycoumarin O-deethylase
Linear with time up to: 12 min
Linear with P450 up to: 10 pmol/ml
Vmax: 0.5 pmol/min/pmol CYP
Km: 1.6 µM
Typical P450 Activity Data (2)
Activity: 7-ethoxyresorufin O-deethylase
Linear with time up to: 1.5 min
Linear with P450 up to: 16 pmol/ml
Vmax: 4 pmol/min/pmol CYP
Km: 0.35 µM
Note: Incubation time 30s for P450 linearity test. Formation of resorufin deviates significantly from linearity at P450 concentrations less than 2 pmol/ml.