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CYP1A1R EasyCYP

Catalogue Number: CYP/EZ014

Product Description: Human CYP1A1 and human CYP-reductase coexpressed in Escherichia coli

Amount: 0.5 nmol cytochrome P450

Storage Buffer: 50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA

Storage: Store at -80°C. Avoid frequent temperature changes. Thaw on ice.

Intended Use: For laboratory (research) purposes only

Typical Batch Characteristics

P450 Concentration: 1.0 nmol/ml

Protein Concentration: 10 mg/ml

Specific P450 Content: 100 pmol/mg protein

Cytochrome c Reductase Activity: 250 nmol/min/mg protein

Typical P450 Activity Data (1)

Activity: 7-ethoxycoumarin O-deethylase

Linear with time up to: 8 min

Linear with P450 up to: 2.5 pmol/ml

Vmax: 20 pmol/min/pmol CYP

Km: 10 µM

Typical P450 Activity Data (2)

Activity: 7-ethoxyresorufin O-deethylase

Linear with time up to: 2 min

Linear with P450 up to: 5 pmol/ml

Vmax: 55 pmol/min/pmol CYP

Km: 0.03 µM


Note: Incubation time 10s for P450 linearity test. Formation of resorufin deviates significantly from linearity at P450 concentrations less than 0.5 pmol/ml.

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