Catalogue Number: CYP/EZ014
Product Description: Human CYP1A1 and human CYP-reductase coexpressed in Escherichia coli
Amount: 0.5 nmol cytochrome P450
Storage Buffer: 50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA
Storage: Store at -80°C. Avoid frequent temperature changes. Thaw on ice.
Intended Use: For laboratory (research) purposes only
Typical Batch Characteristics
P450 Concentration: 1.0 nmol/ml
Protein Concentration: 10 mg/ml
Specific P450 Content: 100 pmol/mg protein
Cytochrome c Reductase Activity: 250 nmol/min/mg protein
Typical P450 Activity Data (1)
Activity: 7-ethoxycoumarin O-deethylase
Linear with time up to: 8 min
Linear with P450 up to: 2.5 pmol/ml
Vmax: 20 pmol/min/pmol CYP
Km: 10 µM
Typical P450 Activity Data (2)
Activity: 7-ethoxyresorufin O-deethylase
Linear with time up to: 2 min
Linear with P450 up to: 5 pmol/ml
Vmax: 55 pmol/min/pmol CYP
Km: 0.03 µM
Note: Incubation time 10s for P450 linearity test. Formation of resorufin deviates significantly from linearity at P450 concentrations less than 0.5 pmol/ml.