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CYP17A1LR Bactosomes (low reductase)

Catalogue Number: CYP065

Product Description: Human CYP17A1 and human CYP-reductase coexpressed in Escherichia coli

Amount: 1 nmol cytochrome P450

Storage Buffer: 50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA

Storage: Store at -80°C. Avoid frequent temperature changes. Thaw on ice.

Intended Use: For laboratory (research) purposes only

Typical Batch Characteristics

P450 Concentration: 1.2 nmol/ml

Protein Concentration: 10 mg/ml

Specific P450 Content:
120 pmol/mg protein

Cytochrome c Reductase Activity:
40 nmol/min/mg protein

Typical P450 Activity Data

Activity: Progesterone 17α-hydroxylase

Linear with time up to: 20 min

Linear with P450 up to: 12.5 pmol/ml

Vmax: 3 pmol/min/pmol CYP

Km: < 0.2 µM

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