Catalogue Number: CYP065
Product Description: Human CYP17A1 and human CYP-reductase coexpressed in Escherichia coli
Amount: 1 nmol cytochrome P450
Storage Buffer: 50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA
Storage: Store at -80°C. Avoid frequent temperature changes. Thaw on ice.
Intended Use: For laboratory (research) purposes only
Typical Batch Characteristics
P450 Concentration: 1.2 nmol/ml
Protein Concentration: 10 mg/ml
Specific P450 Content:
120 pmol/mg protein
Cytochrome c Reductase Activity:
40 nmol/min/mg protein
Typical P450 Activity Data
Activity: Progesterone 17α-hydroxylase
Linear with time up to: 20 min
Linear with P450 up to: 12.5 pmol/ml
Vmax: 3 pmol/min/pmol CYP
Km: < 0.2 µM