Catalogue Number: CYP334
Product Description: Cynomolgus monkey CYP3A4 (see note below) and human CYP-reductase coexpressed in Escherichia coli
Amount: 1 nmol cytochrome P450
Storage Buffer: 50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA
Storage: Store at -80°C. Avoid frequent temperature changes. Thaw on ice.
Intended Use: For laboratory (research) purposes only
Note: In naming this enzyme, we have followed the nomenclature proposed by Uno, Y et al [Drug Metab. Rev. 43 (2011): 346-361]. Earlier publications refer to this enzyme as CYP3A8.
Typical Batch Characteristics
P450 Concentration: 5 nmol/ml
Protein Concentration: 10 mg/ml
Specific P450 Content: 500 pmol/mg protein
Cytochrome c Reductase Activity: 180 nmol/min/mg protein
Typical P450 Activity Data
Activity: Testosterone 6β-hydroxylase
Linear with time up to: 10 min
Linear with P450 up to: 60 pmol/ml
Vmax: 5 pmol/min/pmol CYP
Km: 20 µM