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Cynomolgus CYP2C9LR (CYP2C43) Bactosomes

Catalogue Number: CYP331

Product Description: Cynomolgus CYP2C9 (see note below) and human CYP-reductase coexpressed in Escherichia coli

Amount: 1 nmol cytochrome P450

Storage Buffer: 50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA

Storage: Store at -80°C. Avoid frequent temperature changes. Thaw on ice.

Intended Use: For laboratory (research) purposes only

Note: In naming this enzyme, we have followed the nomenclature proposed by Uno, Y et al [Drug Metab. Rev. 43 (2011): 346-361]. Earlier publications refer to this enzyme as CYP2C43.

Typical Batch Characteristics

P450 Concentration: 2 nmol/ml

Protein Concentration: 8 mg/ml

Specific P450 Content: 250 pmol/mg protein

Cytochrome c Reductase Activity: 180 nmol/min/mg protein

Typical P450 Activity Data

Activity: Testosterone 17-oxidase

Linear with time up to: 20 min

Linear with P450 up to: 30 pmol/ml

Vmax: 12 pmol/min/pmol CYP

Km: 20 µM

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