Catalogue Number: CYP601
Product Description: Polyclonal antiserum raised in sheep against purified rat liver testosterone/4-nitrophenol UGT.
Amount: 50 µl serum
Storage: Store at -20°C. Avoid frequent temperature changes. Thaw on ice.
Intended Use: For laboratory (research) purposes only. A dilution of 1:2500 is suggested for Western blotting applications.
Western blotting specificity (rat UGT):
Each lane contains 20 µg of rat liver microsomal protein.
Lane 1: untreated Wistar
Lane 2: untreated Gunn
Lane 4: 3-methylcholanthrene-treated Wistar
Lane 5: 3-methylcholanthrene-treated Gunn
Lane 7: phenobarbital-treated Wistar
Lane 8: phenobarbital-treated Gunn
Lane 10: beta-naphthoflavone-treated Wistar
Lane 11: beta-naphthoflavone-treated Gunn
Lane 12: clofibrate-treated Wistar
Lane 13: as lane 4
Lanes 3, 6 and 9 are empty
Western blotting specificity (human UGT):
Lane 1: V79 control (30 µg)
Lane 2: UGT1A1 in V79 cells (30 µg)
Lane 3: UGT1A3 in V79 cells (50 µg)
Lane 4: UGT1A6 in V79 cells (30 µg)
Lane 5: UGT1A9 in V79 cells (30 µg)
Lane 6: UGT2B7 in V79 cells (30 µg)
Lane 7: Sf9 control (1 µg)
Lane 8: UGT1A4 in Sf9 cells (1 µg)
Lane 9: human liver microsomes (20 µg)
Immunoinhibition of UGT activity:
There are no data available on the use of this antiserum for the inhibition of UGT activity. IgG prepared from this antiserum has been shown to inhibit UGT activity towards 1-naphthol, testosterone and bilirubin in Lubrol PX-solubilised rat liver microsomes: