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Anti-Rat CYP3A2 Data

Catalogue Number: R-PAP 171

Product Description: Polyclonal antiserum raised in rabbit against purified rat CYP3A2.

Amount: 200 µl serum

Storage: Store at -20°C. Avoid frequent temperature changes. Thaw on ice.

Intended Use: For laboratory (research) purposes only.

Applications: Immunoinhibition and Western blotting.

Typical Batch Characteristics

Specificity of immunoinhibition against rat CYPs:
CYP1A1: –

CYP1A2: –

CYP2B1: –

CYP2B2: –

CYP2C11: –

CYP2E1: –

CYP3A2: ***

CYP4A1: –

***: strong inhibition observed, **: moderate inhibition observed, *: weak inhibition observed, -: no inhibition observed

Potency of inhibition:

Graph showing immunoinhibition of testosterone 6beta-hydroxylation in liver microsomes prepared from a phenobarbital-treated rat. Anti-serum (0 – 50 µl) was pre-incubated with rat liver microsomes (100 µg protein) at room temperature for 30 min before starting the reaction. The final incubation volume was 500 µl. Data published by Daiichi Pure Chemical Co. Ltd.

Blotting Specificity:

CYP1A1: –

CYP1A2: –

CYP2A1: –

CYP2A2: –

CYP2B1: –

CYP2C6: ±

CYP2C11: –

CYP2C13: –

CYP2E1: –

CYP3A2: ***

CYP4A1: –

Degree of cross-reactivity on Western blot: ***: strong, **: moderate, *: weak, -: not detectable

Western blot of rat liver microsomes (1 µg protein), showing the specificity of the R-PAP171 antibody. Data published by Daiichi Pure Chemical Co. Ltd.

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