Cypex Logo

Inhibition Screening

Our inhibition screening service offers fluorimetry based assays to determine the CYP inhibition potential of novel compounds, using bactosomes as the enzyme source.

Our approach ensures cost-effective results that you can rely on.


What you can expect:

  • 8 major human CYPs covered: CYP1A2, CYP2B6, CYP2C8, CYP2C9, CYP2C19, CYP2D6, CYP3A4, and CYP3A5.
  • Dog and pig CYPs covered
  • CYP activity determined at 7 different compound concentrations plus zero and fitted to a model using XLfit® software, before determining an IC50 value.
  • 30-minute incubation time – shifts in IC50 value over time can indicate whether the compound has the potential to be a time-dependent inhibitor of each CYP isoform.
  • Positive control inhibitor and multi-well negative control are run in parallel on every plate, giving confidence in the end result.
  • Recommended solvents are DMSO and methanol up to 2.5% (v/v), assuming compound solubility.
  • Cost effective and fast turnaround of data after receipt of compound.


Download details of the assay


We have recently developed a fluorimetry based inhibition screen for human CYP51, details were presented at the 2019 International ISSX meeting in portland Oregon.  Download the poster

Contact our specialist team for more information

Latest Blogs