Activity: 1-chloro-2,4-dinitrobenzene (CDNB) conjugation
Note: This activity is measured using a continuous spectrophotometric procedure.
Incubation conditions
Assay buffer: 0.1 M potassium phosphate pH 6.5, 1 mM reduced glutathione (GSH)
Incubation volume: 1 ml
Incubation temperature: 37ºC
Detection wavelength: 340 nm