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CYP2J2 QC Assays

Activity: Terfenadine C-hydroxylase

Incubation conditions
Assay buffer: 0.1 M potassium phosphate pH 7.4, 5 mM MgCl2
Incubation volume: 200 µl
Stop reagent: 1 M hydrochloric acid (25 µl)

HPLC conditions
Column: HyperClone 3 µm BDS-C18 125 x 4 mm
Temperature: 40°C
Mobile phase: Water/0.5% trifluoroacetic acid/acetonitrile 38/20/42 (v/v/v; isocratic)
Flow rate: 1.0 ml/min
Run time: 12.8 min
Injection volume: 100 µl
Detection: fluorescence λ(ex) = 230 nm, λ(em) = 290 nm

Retention times:
terfenadine alcohol 2.9 min

The CYP2J2 substrate terfenadine is available from Cypex.

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