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Cynomolgus monkey CYP2C9 (CYP2C43) QC Assays

Activity: Testosterone 17-oxidase

Incubation conditions
Assay buffer: 0.1 M potassium phosphate pH 7.4, 5 mM MgCl2
Incubation volume: 200 µl
Stop reagent: 1 M hydrochloric acid (20 µl)

HPLC conditions
Column: Hypersil ODS (5 µm) 250 x 4 mm
Temperature: 40°C
Mobile phase: 0.05% orthophosphoric acid/methanol 35/65 (v/v) (isocratic separation)
Flow rate: 1.0 ml/min
Run time: 9.0 min
Injection volume: 50 µl
Detection: UV, λ = 240 nm

Retention times:
4-androstene-3,17-dione 5.8 min
testosterone 7.5 min

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