Activity: 4-nitrophenyl butyrate hydrolysis
Note: This activity is measured using a continuous spectrophotometric procedure in a plate reader.
Incubation conditions
Assay buffer: 0.05 M potassium phosphate pH 7.4/acetonitrile 98/2 (v/v)
Incubation volume: 0.25 ml
Incubation temperature: 30ºC
Detection wavelength: 400 nm