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Anti-Rat CYP3A2

 

Catalogue Number: R-PAP 171

Product Description: Polyclonal antiserum raised in rabbit against purified rat CYP3A2.

Amount: 200 µl serum

Storage: Store at -20°C. Avoid frequent temperature changes. Thaw on ice.

Intended Use: For laboratory (research) purposes only.

Applications: Immunoinhibition and Western blotting.

 

Typical Batch Characteristics

Specificity of immunoinhibition against rat CYPs:
 CYP1A1  CYP1A2  CYP2B1  CYP2B2  CYP2C11  CYP2E1  CYP3A2  CYP4A1

-

-

-

-

-

-

***

-
***: strong inhibition observed, **: moderate inhibition observed, *: weak inhibition observed,
-: no inhibition observed

 

Potency of inhibition:

 Graph showing immunoinhibition of rat CYP3A2 by R-PAP171

Graph showing immunoinhibition of testosterone 6beta-hydroxylation in liver microsomes prepared from a phenobarbital-treated rat. Anti-serum (0 - 50 µl) was pre-incubated with rat liver microsomes (100 µg protein) at room temperature for 30 min before starting the reaction. The final incubation volume was 500 µl. Data published by Daiichi Pure Chemical Co. Ltd.

 

Blotting Specificity:

 CYP1A1  CYP1A2  CYP2A1  CYP2A2  CYP2B1  CYP2C6  CYP2C11  CYP2C13

-

-

-

-

-

±

-

-
 CYP2E1  CYP3A2  CYP4A1               

-

***

-

 

 

 

 

 
Degree of cross-reactivity on Western blot: ***: strong, **: moderate, *: weak,
-: not detectable

  .....Western blot of rat liver microsomes using R-PAP171

Western blot of rat liver microsomes (1 µg protein), showing the specificity of the R-PAP171 antibody. Data published by Daiichi Pure Chemical Co. Ltd.

 

Product Information Sheet

 

Important: By purchasing this product from Cypex you accept the standard terms and conditions of supply, details of which may be provided upon request.

 

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