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Anti-Rat CYP1A1

 

Catalogue Number: R-PAP 041

Product Description: Polyclonal antiserum raised in goat against purified rat CYP1A1.

Amount: 200 µl serum

Storage: Store at -20°C. Avoid frequent temperature changes. Thaw on ice.

Intended Use: For laboratory (research) purposes only.

Applications: Immunoinhibition and Western blotting.

 

Typical Batch Characteristics

Specificity of immunoinhibition against rat CYPs:
 CYP1A1  CYP1A2  CYP2B1  CYP2B2  CYP2C11  CYP2E1  CYP3A2  CYP4A1

**

-

-

-

-

-

-

-
***: strong inhibition observed, **: moderate inhibition observed, *: weak inhibition observed,
-: no inhibition observed

 

Potency of inhibition:

 Graph showing immunoinhibition of rat CYP1A1 by R-PAP041

Graph showing immunoinhibition of 7-ethoxycoumarin O-deethylation in liver microsomes prepared from a 3-methylcholanthrene-treated rat. Anti-serum (0 - 50 µl) was pre-incubated with rat liver microsomes (200 µg protein) at room temperature for 30 min before starting the reaction. The final incubation volume was 500 µl. Data published by Daiichi Pure Chemical Co. Ltd.

 

Blotting Specificity:

  .....Western blot of rat liver microsomes using R-PAP041

Western blot of liver microsomes prepared from a 3-methylcholanthrene treated rat (0.3 µg protein), showing the specificity of the R-PAP041 antibody. The band labelled '1' is reported to represent CYP2E1. Data published by Daiichi Pure Chemical Co. Ltd.

 

Product Information Sheet

 

Important: By purchasing this product from Cypex you accept the standard terms and conditions of supply, details of which may be provided upon request.

 

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