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Human CYP3A5R Bactosomes

Catalogue Number: CYP046

Product Description: Human CYP3A5 and human CYP-reductase coexpressed in Escherichia coli

Amount: 1 nmol cytochrome P450

Storage Buffer: 50 mM Tris-acetate (pH 7.6), 250 mM sucrose, 0.25 mM EDTA

Storage: Store at -80°C. Avoid frequent temperature changes. Thaw on ice.

Intended Use: For laboratory (research) purposes only

 

Note: This product is also available in our convenient EasyCYP format.

 

Typical Batch Characteristics

P450 Concentration: 1.5 nmol/ml

Protein Concentration: 10 mg/ml

Specific P450 Content:
      150 pmol/mg protein

Cytochrome c Reductase Activity:
      1200 nmol/min/mg protein

 

Typical P450 Activity Data

Activity: Testosterone 6beta-hydroxylase

Linear with time up to: 5 min

Linear with P450 up to: 20 pmol/ml

Vmax: 60 pmol/min/pmol CYP

Km: 60 µM

 

Product Information Sheets

More Information Available

 

Important: By purchasing this product from Cypex you accept the standard terms and conditions of supply, details of which may be provided upon request.

 

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Made under licence from BTG International Ltd (AU730155, EP0914446, US6566108 and other patents pending).

United States Patent Nos. 5,420,027 or 5,240,831, Canada Patent No. 2100245 and other patents pending.

Linearity with time for CYP3A5R
Linearity with P450 concentration for CYP3A5R

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