Cypex - in vitro drug metabolism systems
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Cypex ALDH1A1 QC Assay

Activity: 4-nitrobenzaldehyde oxidase

Incubation conditions
Assay buffer: 0.05 M Hepes pH 7.4, 1 mM EDTA, 2 mM DTT, 1 mM NAD+
Incubation volume: 500 µl
Stop reagent: 1 M hydrochloric acid (62.5 µl)

HPLC conditions
Column: Gemini C18 (3 µm) 100 x 4.6 mm
Temperature: 60°C
Mobile phase: 0.5% trifluoroacetic acid/methanol/water 40/46/14 (v/v/v) (isocratic)
Flow rate: 1.0 ml/min
Run time: 4.0 min
Injection volume: 100 µl
Detection: UV,
l = 264 nm
Retention times:
       4-nitrobenzaldehyde      2.7 min
       4-nitrobenzoic acid        3.1 min

 

Important: By purchasing this product from Cypex you accept the standard terms and conditions of supply, details of which may be provided upon request.

United States Patent Nos. 4,952,496, 5,693,489 and 5,869,320.

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