Cypex - in vitro drug metabolism systems
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Cypex mouse Cyp3a11 QC Assays

Activity: Testosterone 6b-hydroxylase

Incubation conditions
Assay buffer: 0.05 M potassium phosphate pH 7.4, 5 mM MgCl2
Incubation volume: 200 µl
Stop reagent: 1 M hydrochloric acid (20 µl)
Internal standard: 4-androstene-3,17-dione (10 µl of 10 µg/ml)

HPLC conditions
Column: Hypersil ODS (5 µm) 250 x 4 mm
Temperature: 40°C
Mobile phase: 0.05% orthophosphoric acid/methanol 44/56 (v/v) (isocratic separation)
Flow rate: 1.0 ml/min
Run time: 18.5 min
Injection volume: 50 µl
Detection: UV,
l = 240 nm
Retention times:
      6
b-hydroxytestosterone      5.5 min
      4-androstene-3,17-dione   13.3 min
      testosterone                     17.5 min

 

Made under licence from BTG International Ltd (AU730155, EP0914446, US6566108 and other patents pending).

United States Patent Nos. 5,420,027 or 5,240,831, Canada Patent No. 2100245 and other patents pending.

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